SummaryThe β-aminopeptidase BapA from Sphingosinicella xenopeptidilytica belongs to the N-terminal nucleophile (Ntn) hydrolases of the DmpA-like family and has the unprecedented property of cleaving N-terminal β-amino acid residues from peptides. We determined the crystal structures of the native (αβ)4 heterooctamer and of the 153 kDa precursor homotetramer at a resolution of 1.45 and 1.8 Å, respectively. These structures together with mutational analyses strongly support mechanisms for autoproteolysis and catalysis that involve residues Ser250, Ser288, and Glu290. The autoproteolytic mechanism is different from the one so far described for Ntn hydrolases. The structures together with functional data also provide insight into the discrimina...
γ-Glutamyl transpeptidases (γ-GTs) are members of N-terminal nucleophile hydrolase superfamily. They...
γ-Glutamyl transpeptidases (γ-GTs) are members of N-terminal nucleophile hydrolase superfamily. They...
Linhorst A, Lübke T. The Human Ntn-Hydrolase Superfamily: Structure, Functions and Perspectives. Cel...
The β-aminopeptidase BapA from Sphingosinicella xenopeptidilytica belongs to the N-terminal nucleoph...
AbstractBackground: The l-aminopeptidase d-Ala-esterase/amidase from Ochrobactrum anthropi (DmpA) re...
AbstractBackground: The l-aminopeptidase d-Ala-esterase/amidase from Ochrobactrum anthropi (DmpA) re...
BACKGROUND: The L-aminopeptidase D-Ala-esterase/amidase from Ochrobactrum anthropi (DmpA) releases t...
THE Ntn (N-terminal nucleophile) hydrolases are enzymes with an unusual four-layer alpha \ beta fold...
THE Ntn (N-terminal nucleophile) hydrolases are enzymes with an unusual four-layer alpha \ beta fold...
THE Ntn (N-terminal nucleophile) hydrolases are enzymes with an unusual four-layer alpha \ beta fold...
β-Aminopeptidases are a unique group of enzymes that have the unusual capability to hydrolyze N-term...
The DmpA (d-aminopeptidase A) protein produced by Ochrobactrum anthropi hydrolyses p-nitroanilide de...
N-terminal nucleophile (Ntn)-hydrolases catalyze the cleavage of amide bonds in a variety of macromo...
Plant l-asparaginases and their bacterial homologs, such as EcAIII found in Escherichia coli, form a...
The Ntn (N terminal nucleophile) hydrolases are a new family of hydrolytic enzymes with a characteri...
γ-Glutamyl transpeptidases (γ-GTs) are members of N-terminal nucleophile hydrolase superfamily. They...
γ-Glutamyl transpeptidases (γ-GTs) are members of N-terminal nucleophile hydrolase superfamily. They...
Linhorst A, Lübke T. The Human Ntn-Hydrolase Superfamily: Structure, Functions and Perspectives. Cel...
The β-aminopeptidase BapA from Sphingosinicella xenopeptidilytica belongs to the N-terminal nucleoph...
AbstractBackground: The l-aminopeptidase d-Ala-esterase/amidase from Ochrobactrum anthropi (DmpA) re...
AbstractBackground: The l-aminopeptidase d-Ala-esterase/amidase from Ochrobactrum anthropi (DmpA) re...
BACKGROUND: The L-aminopeptidase D-Ala-esterase/amidase from Ochrobactrum anthropi (DmpA) releases t...
THE Ntn (N-terminal nucleophile) hydrolases are enzymes with an unusual four-layer alpha \ beta fold...
THE Ntn (N-terminal nucleophile) hydrolases are enzymes with an unusual four-layer alpha \ beta fold...
THE Ntn (N-terminal nucleophile) hydrolases are enzymes with an unusual four-layer alpha \ beta fold...
β-Aminopeptidases are a unique group of enzymes that have the unusual capability to hydrolyze N-term...
The DmpA (d-aminopeptidase A) protein produced by Ochrobactrum anthropi hydrolyses p-nitroanilide de...
N-terminal nucleophile (Ntn)-hydrolases catalyze the cleavage of amide bonds in a variety of macromo...
Plant l-asparaginases and their bacterial homologs, such as EcAIII found in Escherichia coli, form a...
The Ntn (N terminal nucleophile) hydrolases are a new family of hydrolytic enzymes with a characteri...
γ-Glutamyl transpeptidases (γ-GTs) are members of N-terminal nucleophile hydrolase superfamily. They...
γ-Glutamyl transpeptidases (γ-GTs) are members of N-terminal nucleophile hydrolase superfamily. They...
Linhorst A, Lübke T. The Human Ntn-Hydrolase Superfamily: Structure, Functions and Perspectives. Cel...