Hyper production of cellulose degrading endo (1,4) β-d-glucanase from Bacillus licheniformis KIBGE-IB2

AbstractCellulase hydrolyzes β (1,4) glycosidic linkages of cellulose polymer to soluble sugar. An extracellular enzyme production by Bacillus licheniformis KIBGE-IB2 (GenBank accession No. GU216259) was studied under various environmental conditions. Maximum enzyme production was measured in the liquid fermentation medium after 48 h, containing (gL−1), CMC, 5.0; peptone, 15.0; yeast extract, 15.0; CaCl2·2H2O, 0.001; FeSO4·7H2O, 0.001; K2HPO4, 5.0; NaH2PO4, 5.0 and MgSO4·7H2O, 1.0. The optimal pH and temperature for enzyme production was found to be 6.0 and 37°C, respectively. It was also found that beside soluble sugars, a significant amount of enzyme production was obtained when biomass (wheat bran and orange peel) were examined as a sole carbon source. The current findings indicate that endo (1,4) β-d-glucanase from B. licheniformis KIBGE-IB2 can be beneficial for commercial purpose