Biphasic Effect of 1,25-Dihydroxyvitamin D3 on Human Hair Follicle Growth and Hair Fiber Production in Whole-Organ Cultures

We have used a whole-organ culture system to investigate the effects of 1,25(OH)2 D3 on human hair follicle growth and hair fiber production. Relatively low concentrations (1-10 nM) of 1,25(OH)2D3 stimulated the cumulative growth of hair follicles and hair fibers, by 52% and 36%, respectively (concentration producing 50% of the maximal response [EC50] values of 0.3 nM). The initial rates of follicle and fiber growth were increased, whereas the respective growth periods were unaffected. At higher concentrations of 1,25(OH)2D3, there was a dose-dependent inhibition of both follicle and fiber growth (IC50 values of 100 nM), in pan due to reduction in the growth periods. There was a marked delay between the onset of 1,25(OH)2D3-induced hair follicle and hair fiber growth inhibition. Incubation of hair follicles with 100nM 1,25(OH)2D3 resulted in a rapid, transient inhibition of DNA synthesis (55% inhibition at 24 h), followed by a gradual return to control levels at day 4. Prolonged (>5 h), incubation in the presence of 100nM of 1,25(OH)2D3 was required for follicle growth inhibition to be manifest. Ro 31-7549, a selective inhibitor of protein kinase C, did not prevent 1,25(OH)2D3-induced inhibition of hair follicle growth. These data suggest that 1,25(OH)2D3 may play a physiologic role in maintaining optimal hair follicle activity, and that elevation of 1,25(OH)2D3 may inhibit hair growth in vivo