Direct effects of Daphnia-grazing, not infochemicals, mediate a shift towards large inedible colonies of the gelatinous green alga Sphaerocystis schroeteri

AbstractThe influence of Daphnia galeata×hyalina grazing and of infochemicals released by the daphnids on the colony size and growth rate of the colonial gelatinous green alga Sphaerocystis schroeteri (Chlorococcales) was investigated in laboratory batch experiments run for 96h. High zooplankton grazing pressure was exerted by a final concentration of 100daphnidsL−1 in the Daphnia treatments. Infochemicals were obtained by filtration (0.2μm) of water from D. galeata×hyalina cultures (200ind.L−1 exposed for 24h). This filtrate was added to the S. schroeteri cultures in two concentrations corresponding to 7 and 50daphnidsL−1, respectively. The growth rate of S. schroeteri was neither affected significantly by direct Daphnia grazing nor by the presence of Daphnia infochemicals, in comparison to the control. However, the portion of inedible S. schroeteri colonies (diameter>50μm) increased under direct grazing pressure, whereas the Daphnia infochemicals did not influence the colony size significantly. We conclude that the shift in colony size by direct zooplankton grazing denotes an effective defence mechanism against size selective feeding for colonial gelatinous green algae. This effective defence in combination with unchanged growth rates of the larger colonies (under non-limiting nutrient and light conditions) falsifies the assumption of a trade-off between minimising grazing losses and maximising growth by optimising the colony size