Effect of fatty acid anilides on the generation of arachidonic acid by human polymorphonuclear leukocytes

AbstractThe addition of oleoylanilide or linoleylanilide to human polymorphonuclear leukocytes induces a time-and dose-dependent generation of arachidonic acid. Half-maximal effect is caused by a dose of 0.2 mg linoleylanilide/ml. Fatty acid anilides also produce a time- and dose-dependent inhibition of the synthesis of triacylglycerol. Half-maximal effect is caused by 1 μg linoleylanilide/ml. These results indicate that fatty acid anilides, which have been found in the illegal cooking oil which intoxicated thousands of Spaniards, alter lipid metabolism in human polymorphonuclear leukocytes