The melanocortin (MC3) receptor from rat hypothalamus: Photoaffinity labelling and binding of alanine-substituted α-MSH analogues

AbstractMembrane preparations of cells expressing the cloned rat hypothalamus melanocortin receptor, MC3, have been photoaffinity labelled using a radiolabelled photoreactive analogue of α-MSH, [125I-Tyr2,Nle4,d-Phe7,ATB-Lys11]α-MSH.SDS-PAGE followed by autoradiography showed a single band at 53–56 kDA for the native receptor of 35 kDA after deglycosylated with PNGase F, consistent with the predicted cDNA. Receptor binding studies with α-MSH, γ-MSH and [Nle4,d-Phe7]α-MSH established that α-MSH and γ-MSH had similar affinities while [Nle4,d-Phe7]α-MSH bound 100 times more strongly. These results suggest that the receptor recognises the conserved ‘core sequence’ (-Met-Glu/Gly-His-Phe-Arg-Trp-) of MSH/ACTH peptides. The binding affinities of alanine-substituted analogues of α-MSH were determined to investigate the role of individual residues in ligand—receptor interactions. While in the terminal regions only the replacement of Tyr2 reduced the affinity of the peptide, replacement of Met4, Phe7, Arg8 and Trp9 within the peptide core led to a significant loss of affinity. Glu5 appeared unimportant for receptor recognition