A new method to discriminate between enzyme-kinetic models

AbstractA novel statistical procedure [S. Zwanzig, Math. Opsforsch. Statist. Ser. Statist.11, 23–47 (1980)] is applied to discriminate between kinetic models of enzyme reactions. Two examples are elucidated on the basis of numerically simulated data: 1.(a) Discrimination between a Michaelis-Menten-model and a Hill-equation2.(b) Discrimination between competitive and noncompetitive inhibition of a two-substrate reaction.Next the proposed method is applied to assess a kinetic model of the allosteric enzyme phosphofructokinase from the malarial parasite Plasmodium berghei. It is demonstrated that the novel procedure allows a more sensitive discrimination as it is obtained with alternative statistical tests