Optimization of Glycosaminoglycan Extraction on Patinopecten Yessoensis Waste

AbstractOn the basis of single factor experiment, the pH of enzymatic hydrolysis, enzymolysis temperature, enzymolysis time and solid-liquid ratio as independent variable, extraction rate as response value, extraction technology of glycosaminoglycan from Patinopecten yessoensis waste were optimized using response surface methodology. The order affecting glycosaminoglycan extraction rate was determined: the enzymatic pH > solid-liquid ratio > enzymatic time > enzymatic temperature. The optimal conditions of extraction were: the pH of enzymatic hydrolysis was 8.0, enzymolysis temperature was 40°C, enzymolysis time was 3.5h and solid-liquid ratio was 1:2. Click here and insert your abstract text