dam methylase from E. coli Circular dichroism investigations of the secondary structure and influence of S-adenosylmethionine

AbstractThe enzyme dam methylase which recognizes and methylates the adenine in the palindromic sequence GATC in DNA was isolated and the secondary structure was determined by CD spectroscopy and various predicting methods from the amino acid sequence. The interaction of dam methylase with S-adenosylmethionine was studied by CD spectroscopy indicating a decrease of the percentage of α-helix as the amount of S-adenosylmethionine bound to the enzyme was increased