Interleukin-12 gene adjuvant increases the immunogenicity of virus-like particles of human papillomavirus type 16 regional variant strain
- Wei, Lanlan
- Chu, Ming
- Zhang, Qingmeng
- Wang, Yan
- Shang, Qinglong
- Zhang, Yunyan
- Zhang, Guangmei
DOIAbstract
AbstractObjectivesTo analyze the immunogenicity of virus-like particles (VLP) of human papillomavirus type 16 (HPV16) isolated in East China and the adjuvant potential of interleukin-12 (IL-12).MethodsThe variant HPV16 L1VLP expressed in sf9 insect cells were purified with cesium chloride gradient centrifugation. BALB/c mice were vaccinated with VLP (L1N), VLP with Freund's adjuvant (L1A) or VLP with IL-12 recombinant plasmid (L1P). HPV16 VLP specific IgG and IFN-γ level in the serum were detected by ELISA, and the percentage of CD4+ and CD8+ in spleen cells was detected with flow cytometry.ResultsThe titers of serum IgG antibodies in vaccinated groups were higher than in negative control and the serum antibodies mainly recognized conformat...
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