SummaryReprogramming of somatic cells achieved by combination of the four transcription factors Oct4, Sox2, Klf4, and c-Myc has very low efficiency. To increase the reprogramming efficiency and better understand the process, we sought to identify factors that mediate reprogramming with higher efficiency. We established an assay to screen nuclear fractions from extracts of pluripotent mouse cells based on Oct4 reactivation. Using proteomics, we identified components of the ATP-dependent BAF chromatin-remodeling complex, which significantly increases reprogramming efficiency when used together with the four factors. The reprogrammed cells could transmit to the germline and exhibited pluripotency. Reprogramming remained highly efficient when c...
SummaryEctopic expression of the transcription factors Oct4, Sox2, c-Myc, and Klf4 in fibroblasts ge...
Ectopically expressing the transcription factors (TFs) Oct4, Sox2, Klf4, and c-Myc (OSKM) leads to t...
Cellular differentiation involves profound remodelling of chromatic landscapes, yet the mechanisms b...
Induced pluripotent stem cells (iPSCs) have been produced from a number of mouse and human somatic c...
© Copyright 2015, Mary Ann Liebert, Inc. 2015. The SWI/SNF (SWItch/Sucrose NonFermentable or BAF, Br...
Despite intensive efforts to optimize the process, reprogramming differentiated cells to induced plu...
Significant breakthroughs have been recently achieved in reprogramming somatic cells to a pluripoten...
SummarySomatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) with the introduc...
AbstractUnderstanding the molecular underpinnings of pluripotency is a prerequisite for optimal main...
Summary: Despite intensive efforts to optimize the process, reprogramming differentiated cells to in...
Oct4, Sox2, Klf4, and cMyc (OSKM) reprogram somatic cells to pluripotency. To gain a mechanistic und...
Resetting differentiated cells to a pluripotent state is now a widely applied technology and a key s...
Patient-specific somatic cell reprogramming is likely to have a large impact on medicine by providin...
Somatic cell reprogramming into induced pluripotent stem cells (iPSCs) induces changes in genome arc...
Ectopic expression of four transcription factors, Oct4, Sox2, Klf4, and c-Myc, coverts somatic cells...
SummaryEctopic expression of the transcription factors Oct4, Sox2, c-Myc, and Klf4 in fibroblasts ge...
Ectopically expressing the transcription factors (TFs) Oct4, Sox2, Klf4, and c-Myc (OSKM) leads to t...
Cellular differentiation involves profound remodelling of chromatic landscapes, yet the mechanisms b...
Induced pluripotent stem cells (iPSCs) have been produced from a number of mouse and human somatic c...
© Copyright 2015, Mary Ann Liebert, Inc. 2015. The SWI/SNF (SWItch/Sucrose NonFermentable or BAF, Br...
Despite intensive efforts to optimize the process, reprogramming differentiated cells to induced plu...
Significant breakthroughs have been recently achieved in reprogramming somatic cells to a pluripoten...
SummarySomatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) with the introduc...
AbstractUnderstanding the molecular underpinnings of pluripotency is a prerequisite for optimal main...
Summary: Despite intensive efforts to optimize the process, reprogramming differentiated cells to in...
Oct4, Sox2, Klf4, and cMyc (OSKM) reprogram somatic cells to pluripotency. To gain a mechanistic und...
Resetting differentiated cells to a pluripotent state is now a widely applied technology and a key s...
Patient-specific somatic cell reprogramming is likely to have a large impact on medicine by providin...
Somatic cell reprogramming into induced pluripotent stem cells (iPSCs) induces changes in genome arc...
Ectopic expression of four transcription factors, Oct4, Sox2, Klf4, and c-Myc, coverts somatic cells...
SummaryEctopic expression of the transcription factors Oct4, Sox2, c-Myc, and Klf4 in fibroblasts ge...
Ectopically expressing the transcription factors (TFs) Oct4, Sox2, Klf4, and c-Myc (OSKM) leads to t...
Cellular differentiation involves profound remodelling of chromatic landscapes, yet the mechanisms b...