249 Validation of assessment of circulate oxidative stress markers by the Free Oxygen Radicals Testing (FORT) assay among patients with an acute myocardial infarction

BackgroundFree oxygen radicals play an important role in the pathogenesis of many diseases including cardiovascular diseases, diabetes, cancer and aging. Several methods were developed for the direct or indirect measurement of oxygen free radical and its by-products. Using a new Free Oxygen Radicals Testing (FORT) the current study is designed first to validate the device and to investigate the potential relationships between the ROS and clinical or biological factors in human serum from a population of men with an acute myocardial infarction (AMI).MethodsWe first determined the effect of storage, variability and reproducibility of the FORT test in serum. Then we used the test in 66 patients from our bio bank of AMI patients.ResultsFORT values vary between 324 and 1198 FORT units, with a median value of 581 (494–754) FORT units. Among the risk factors, 17% of patients are diabetic, and 20% are obese. In univariate analysis, the FORT values seem to be influenced by age (r = 0.161, p = 0.195), presence of diabetes (p = 0.102), a history of MI (p = 0.181), LVEF <40% (p = 0.005) and treatment with - blockers before admission (p = 0.053), with ST-Elevation MI (p = 0.058), levels of CRP (r = 0.438, p<0.001), the rate of neutrophil (r = 0.203, p = 0.107) and peak CK (r = 0.274, p = 0.028). The analysis of multiple linear regression showed that CRP (p = 0.023), LVEF <40% (p