Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato
- Shetty, Deeti K.
- Inamdar, Maneesha S.
Publication date
March 2016
DOI Publisher
University of Texas at Austin Dell Medical School. Published by Elsevier B.V.Abstract
AbstractHuman embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity within individual cells in hESC colonies by enabling live tracking of their growth, migration and differentiation properties. This cell line also serves as a resource for additional transgene introduction/knock-out/knock-in generation in a fluorescent background and allows ease of analysis in studies involving cell mixing
Add to ORKG