Identification of messenger RNA for human type II collagen

AbstractTotal RNA was purified from human fetal calvaria and articular cartilage. Messenger RNAs for type I and II collagens were identified by hybridization using cDNA clones for chicken proα1(I)-, proα2(I)- and proα1(II)collagen mRNAs and by analysis of cell-free translation products of these RNAs by polyacryl-amide gel electrophoresis. The size of human proα1(II)collagen mRNA is approx. 5100 bases. Translatability of cartilage specific type II collagen mRNA was found to be concentration dependent: with increasing total RNA concentrations the relative translation of type II collagen mRNA was reduced with respect to type I mRNAs.CollagenHuman type II collagenCartilageHybridizationmRNACell-free translatio