Optimization Lactic Acid Production from Molasses Renewable Raw Material through Response Surface Methodology with Lactobacillus Casei M-15

AbstractLactic acid is one of the functional and valuable compounds utilized in food, pharmaceutical and chemical industries while Poly lactic acid (PLA) is a biodegradable polymer that has a variety of applications. In recent years, microbial conversion of renewable raw materials has become an important objective in industrial biotechnology. Sugarcane molasses can be considered as potential renewable raw materials in PLA production. The objective of this study is to optimized fermentation medium and conditions to obtain maximum lactic acid production and Colony Forming Unit (log CFU/mL) through response surface methodology (RSM). The maximum lactic acid production (38.33%) and log CFU/mL (8.30) by Lactobacillus casei M-15 was under 3.82% of molasses and 8.02% of inoculum level within 24 hr at 37°C respectively. This process will be advantageous for increasing yields of lactic acid and enhancing productivity by optimization technique. Moreover, it can reduce waste disposal and pollution and can selectively produce by sustainable agriculture such as agriculture material. In addition, the high-performance of lactic acid-producing microorganisms, qualified renewable raw materials and effective fermentation processes will be benefit for bioplastic technologies