Polymerase chain reaction is a recently developed procedure to detect point mutations in human genomic DNA. The sensitivity and specificity of this technique which requires a very little amount of DNA, have allowed the large scale screening of tumors. The Authors report the detection of point mutations of Ha-ras gene at codon 12 in 12 basal cell carcinomas and 10 cutaneous squamous cell carcinomas by the use of polymerase chain reaction in paraffin-embedded tissue sections. The gene alteration was identified in 2 basaliomas whereas no squamous cell carcinoma showed point mutations. These results suggest that point mutations of Ha-ras gene do not represent a general activating mechanism in cutaneous epitheliomas
CONETXT: Ras gene mutations have been associated to a wide range of human solid tumors. Members of t...
Codon 12 of the ras protooncogenes represents a mutational hotspot in human cancer. A distinct patte...
We have used a rapid, non-radioactive and sensitive method based on allele-specific amplification us...
Detection of point mutations of Ha-ras gene in basalimas and spinaliomas by the use of polymerase ch...
Activated ras oncogenes have been detected in a variety of human malignancies. Activation of ras onc...
The human ras gene plays a fundamental role in the transduction of extracellular signals to the nucl...
Previous studies have demonstrated that genes of the ras family (H, K, and N) can be activated by po...
Precise data on the incidence of transforming ras oncogenes in pediatric tumors and the correlations...
Precise data on the incidence of transforming ras oncogenes in pediatric tumors and the correlations...
Previous studies have shown that c-Ha-ras-1 and other genes located on the short arm of chromosome 1...
Mutations within codon 12 leading to activation of Kirsten-ras (K-ras) genes occur in a wide variety...
In an attempt to define genomic copy number changes associated with the development of basal cell ca...
In this study we address whether there is an association between ras mutations and disease progressi...
In an attempt to define genomic copy number changes associated with the development of basal cell ca...
There is strong evidence that the patched (PTCH) gene is a gene for susceptibility to the nevoid bas...
CONETXT: Ras gene mutations have been associated to a wide range of human solid tumors. Members of t...
Codon 12 of the ras protooncogenes represents a mutational hotspot in human cancer. A distinct patte...
We have used a rapid, non-radioactive and sensitive method based on allele-specific amplification us...
Detection of point mutations of Ha-ras gene in basalimas and spinaliomas by the use of polymerase ch...
Activated ras oncogenes have been detected in a variety of human malignancies. Activation of ras onc...
The human ras gene plays a fundamental role in the transduction of extracellular signals to the nucl...
Previous studies have demonstrated that genes of the ras family (H, K, and N) can be activated by po...
Precise data on the incidence of transforming ras oncogenes in pediatric tumors and the correlations...
Precise data on the incidence of transforming ras oncogenes in pediatric tumors and the correlations...
Previous studies have shown that c-Ha-ras-1 and other genes located on the short arm of chromosome 1...
Mutations within codon 12 leading to activation of Kirsten-ras (K-ras) genes occur in a wide variety...
In an attempt to define genomic copy number changes associated with the development of basal cell ca...
In this study we address whether there is an association between ras mutations and disease progressi...
In an attempt to define genomic copy number changes associated with the development of basal cell ca...
There is strong evidence that the patched (PTCH) gene is a gene for susceptibility to the nevoid bas...
CONETXT: Ras gene mutations have been associated to a wide range of human solid tumors. Members of t...
Codon 12 of the ras protooncogenes represents a mutational hotspot in human cancer. A distinct patte...
We have used a rapid, non-radioactive and sensitive method based on allele-specific amplification us...