Extraction of Ethanolic Extract of Red Betel Leaves and Its Cytotoxicity Test on HeLa Cells

AbstractExtraction and identification of active compounds in red betel leaves (Piper crocatum Ruiz&Pav.) have been conducted. Ethanolic extract of red betel leaves was obtained by Soxhlet extraction followed by solvent removal step using rotary evaporator. Compound identification of the extract was performed by GCMS. The concentrated ethanol extract was then examined through cytotoxicity test on Hela cells. Results from GCMS spectrum depicted several compounds which were identified in the extract, i.e. neophytadiene, elimicin and propionic acid. Cytotoxicity test was conducted on Hela cells using in vitro method and calculated using Probit method. LC50 value from the ethanolic extract of red betel leaf was 0.81 + 0.26mg/ml. This result showed that the bioactive compounds potentially inhibited proliferation of Hela cells