A comparison of the initiating abilities of ribo- and deoxyriboprimers in DNA polymerization catalyzed by AMV reverse transcriptase
- Lokhova, I.A.
- Nevinsky, G.A.
- Gorn, V.V.
- Veniaminova, A.G.
- Repkova, M.V.
- Kavsan, V.M.
- Rudenko, N.K.
- Lavrik, O.I.
DOIAbstract
AbstractThe difference in optimal conditions for DNA polymerization catalyzed by AMV reverse transcriplase on poly(A) and poly(dA) templates with d(pT)10 and (pU)10 primers has been found. A comparison of the initiating abilities of d(pT)10 and (pU)10, primers under optimal conditions for various template · primer complexes has been made. The best template · primer complex was poly(A) · d(pT)10 and the worst was poly(A) · (pU)10. The lengthening of d (pT)n primers by a mononucleotide unit (n=2 10) increases their affinity by a factor of about 2 and 3 in the case of poly(dA) and poly(A) templates, respectively. The affinities ofd (pT)11–25 to the enzyme does not change with the primer length
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