Effective method for obtaining long nucleotide chains on partially complementary templates Processed bovine γ-interferon gene obtained from human γ-interferon gene
- Rostapshov, V.M.
- Chernov, I.P.
- Azhikina, T.L.
- Borodin, A.M.
- Sverdlov, E.D.
DOIAbstract
AbstractThe method of obtaining the bovine γ-interferon gene by means of simultaneous multidirected mutagenesis of the human γ-interferon gene is presented. The first strand of the bovine γ-interferon gene was obtained by ligation of synthetic oligonucleotides, using the cDNA of human γ-interferon, cloned in the single-stranded phage M13mpl9 as a template. The second strand was synthesized using a large fragment of E. coli DNA-polymerase 1. The double-stranded gene was then treated by restriction nucleases and cloned in a pUC- 18 derived vector. The primary structure was confirmed by sequencing
Add to ORKG![Ovine interferon-<img src='/image/spc_char/gamma2.gif'> gene of indigenous sheep: Cloning, sequencing and expression studies in <i style="">Escherichia coli</i>](/en/item/297940941/Ovine-interferon-lessimg-src'imagespc_chargamma2.gif'greater-gene-of-indigenous-sheep:-Cloning-sequencing-and-expression-studies-in-lessi-style""greaterEscherichia-colilessigreater){kind=link}