Comparison of the In Vivo and In Vitro Percutaneous Absorption of a Lipophilic Molecule (Cypermethrin, a Pyrethroid Insecticide)

The absorption of a pyrethroid insecticide, cypermethrin, through rat skin has been measured both in vitro and in vivo. Cypermethrin did not penetrate in vitro through whole skin but did penetrate epidermal membranes. The in vitro absorption was influenced by the choice of receptor fluid in the glass diffusion cell. There was good agreement between in vivo and in vitro data using 50% aqueous ethanol, 6% Volpo 20, or total calf serum receptor fluids. Rat epidermal membranes in vitro were more than 20 times more permeable to cypermethrin than human epidermal membranes, indicating that cypermethrin would be less readily absorbed in humans than in the rat. The percutaneous absorption in vitro technique using epidermal membranes was successfully used with this Iipophilic chemical to predict the in vivo absorption in the rat