New Bioinsecticide Granules Toxin from Ectract of Papaya (Carica Papaya) Seed and Leaf Modified Against Aedes Aegypti Larvae

AbstractThe epidemic of dengue hemorrhagic fever (DBD) in Indonesia, which occurs every year, has become a problem for the Indonesian society. Aedes aegypti mosquito is a vector of this disease. During this time the larvae of Aedes aegypti only has one exterminator is abate. In the long time exposure of single type insecticide will cause resistance. Therefore, it is necessary to find new bioinsectiside which is expected to have effects such as abate. The aims of this study are to offer technology, dosage, formulation and toxicity of new bioinsecticide granules toxin from ectract of papaya (Carica papaya) seed and leaf modified against Aedes aegypti larvae.Methodpreparation of the extract by percolation method with 70% ethanol for seeds, and with water-ethanol 70% for leaf. Bioassay with active ingredients with thin layer chromatography. Formulation for 300g Granules are: active substances papaya seed and leaf modified: 90%, seed flour 5%, filler: 5%. This was an experiment using a completely randomized design with 6 treatment groups. Each group contained 20 third instar larvae of Aedes aegypti. Concentrations of granules are 0, 30, 60, 90. 120, 150ppm, with Temephos 1% as a positive control. Data was analyzed using Probit analysis.ResultsThe result of phytochemical analysis contains secondary metabolites compounds of saponin, flavonoid and triterpenoid. Toxicity (LC50) in this study is reported 107ppm; 48hours and LC90: 150ppm, 48hours.ConclusionNew Bioinsecticide Granules Toxin from extract of Papaya (Carica papaya) Seed and leaf Modified Against Aedes aegypti larvae possess technology, dosage, formulation, and larvicidal remarkable. Further investigations are needed to elucidate active ingredient responsible for larvacidal activity should be identified and utilized in preparing a commercial product