Integrin α2β1 Mediates the Cell Attachment of the Rotavirus Neuraminidase-Resistant Variant nar3

AbstractIt was previously reported that integrins α2β1, α4β1, and αXβ2 are involved in rotavirus cell infection. In this work we studied the role of integrin subunits α2, α4, and β2 on the attachment of rotaviruses RRV and nar3 to MA104 cells. Integrin α2β1 was found to serve as the binding receptor for the neuraminidase-resistant virus nar3, whereas the neuraminidase-sensitive strain RRV interacted with this integrin at a postattachment step. It was shown that nar3 binds α2β1 through the DGE integrin-recognition motif located in the virus surface protein VP5. Integrin subunits α4 and β2 do not seem to be involved in the initial cell binding of either virus