Multiplex Diagnosis of Oncogenic Fusion and MET Exon Skipping by Molecular Counting Using Formalin-Fixed Paraffin Embedded Lung Adenocarcinoma Tissues
- Sunami, Kuniko
- Furuta, Koh
- Tsuta, Koji
- Sasada, Shinji
- Izumo, Takehiro
- Nakaoku, Takashi
- Shimada, Yoko
- Saito, Motonobu
- Nokihara, Hiroshi
- Watanabe, Shun-ichi
- Ohe, Yuichiro
- Kohno, Takashi
DOIAbstract
AbstractIntroductionFusions of the anaplastic lymphoma receptor tyrosine kinase gene (ALK), ret proto-oncogene (RET), ROS proto-oncogene 1, receptor tyrosine kinase gene (ROS1), B-Raf proto-oncogene, serine/threonine kinase gene (BRAF), and neuregulin 1 gene (NRG1) and intronic MMNG HOS Transforming gene (MET) mutations are druggable oncogene alterations in lung adenocarcinoma that cause expression of aberrant transcripts. Because these aberrant transcripts are both infrequent (incidence <5%) and mutually exclusive, multiplex assays are required to detect them in tumor samples.MethodsAberrant transcripts of the six aforementioned oncogenes (36 transcripts in total) were examined in a molecular counting (MC) assay, which counts RNA molecules...
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