PapG II adhesin in the establishment and persistence of Escherichia coli infection in mouse kidneys
- Tseng, C.C.
- Huang, J.J.
- Wang, M.C.
- Wu, A.B.
- Ko, W.C.
- Chen, W.C.
- Wu, J.J.
DOIAbstract
Most human pyelonephritogenic Escherichia coli express the PapG II adhesin. However, the role of the PapG II adhesin in enhancing the establishment and persistence of E. coli infection in the kidney is controversial. A pyelonephritogenic strain, EC114, which possesses one copy of the papG II gene, but without other virulence factors (such as S/F1C-fimbriae, hemolysin, and cytotoxic necrotizing factor 1) was selected for the construction of a papG II mutant. The resulting papG II mutant was confirmed by polymerase chain reaction, Southern hybridization, and agglutination assay, and designated as MEC114. We compared MEC114 with the parental strain (EC114) for colonization ability in the bladder and kidney of female BALB/c mice, which were cha...
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