AbstractTwo-level lambda-calculus is designed to provide a mathematical model of capturing substitution, also called instantiation. Instantiation is a feature of the ‘informal meta-level’; it appears pervasively in specifications of the syntax and semantics of formal languages.The two-level lambda-calculus has two levels of variable. Lambda-abstraction and beta-reduction exist for both levels. A level 2 beta-reduct, triggering a substitution of a term for a level 2 variable, does not avoid capture for level 1 abstractions. This models meta-variables and instantiation as appears at the informal meta-level.In this paper we lay down the syntax of the two-level lambda-calculus; we develop theories of freshness, alpha-equivalence, and beta-reduc...