Selective inhibition of CYP27A1 and of chenodeoxycholic acid synthesis in cholestatic hamster liver
- Matsuzaki, Yasushi
- Bouscarel, Bernard
- Ikegami, Tadashi
- Honda, Akira
- Doy, Mikio
- Ceryak, Susan
- Fukushima, Sugano
- Yoshida, Shigemasa
- Shoda, Junichi
- Tanaka, Naomi
DOIAbstract
AbstractThe aim of this study was to explore the regulation of serum cholic acid (CA)/chenodeoxycholic acid (CDCA) ratio in cholestatic hamster induced by ligation of the common bile duct for 48 h. The serum concentration of total bile acids and CA/CDCA ratio were significantly elevated, and the serum proportion of unconjugated bile acids to total bile acids was reduced in the cholestatic hamster similar to that in patients with obstructive jaundice. The hepatic CA/CDCA ratio increased from 3.6 to 11.0 (P<0.05) along with a 2.9-fold elevation in CA concentration (P<0.05) while the CDCA level remained unchanged. The hepatic mRNA and protein level as well as microsomal activity of the cholesterol 7α-hydroxylase, 7α-hydroxy-4-cholesten-3-one 1...
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