Automated nanospray using chip-based emitters for the quantitative analysis of pharmaceutical compounds
- Corkery, Leonard J.
- Pang, Henrianna
- Schneider, Bradley B.
- Covey, Thomas R.
- Siu, K.W. Michael
Publication date
March 2005
DOI Publisher
American Society for Mass Spectrometry. Published by Elsevier B.V.Abstract
An automated nanospray system based on chip technology (the NanoMate) was successfully interfaced to a modified Particle Discriminator Interface on a triple quadrupole mass spectrometer. A number of the interface parameters were optimized to improve the sampling efficiency for ions from the chip-based system. Analytical performance was assessed using a number of biochemicals as well as via a methodology for a pharmaceutical that passed validation as required by Good Laboratory Practices. Infusion analyses in flow rates <1 μL/min provided advantages in terms of throughput and sample consumption when compared to other methodologies based on liquid chromatography