AbstractMethylation of cytosine in the DNA inhibits the transcription by RNA polymerase II in higher eukaryotes, but has no influence on RNA polymerase I transcription. The effect on RNA polymerase III was unknown, so far. Two polymerase III genes: a type 1 5S rRNA gene and a type 2 tRNA gene were methylated in vitro with a purified eukaryotic DNA methyltransferase (EC2.1.1.37) and their transcription was analyzed in Xenopus oocytes. The 5S rRNA gene, an oocyte 5S rRNA gene from X. laevis which is subject to developmental inactivation, was not affected by methylation. Conversely, transcription of the tRNA gene was 80% inhibited by methylation with the eukaryotic methyltransferase. HhaI and HpaII methylation left its transcription unaffected
g.oxfordjournals.org/ D ow nloaded from 2 Despite the fact that 45 % of all human gene promoters do ...
International audienceDouble-stranded RNAs (dsRNAs) and their 'diced' small RNA products can guide k...
RNA polymerase III (Pol III) as well as Pol II (35S) promoters are able to drive hairpin RNA (hpRNA)...
AbstractMethylation of cytosine in the DNA inhibits the transcription by RNA polymerase II in higher...
AbstractMethylation of cytosine-residues in the sequence CpG affects the expression of many genes an...
The use of nucleases to probe the higher-order organisation of genes and the development of cell-fre...
International audienceMethylation has often been correlated with transcriptional inhibition of genes...
Mammalian genomes contain huge numbers of short interspersed elements (SINEs). An extreme case is p...
The methylation sensitive restriction enzymes Hha I and Hpa II were used to analyse the methylation ...
[[abstract]]In this communication we identify and initially characterize two antagonistic activities...
Summary A hallmark of vertebrate genes is that actively transcribed genes are hypomethylated in crit...
Epigenetic gene silencing results from the inhibition of transcription or from posttranscriptional R...
Eukaryotic genomes contain 5-methylcytosine (5mC) as a rare base. 5mC arises by postsynthetic modifi...
AbstractMany RNA polymerase II- or III-transcribed genes are inactive when their promoter is methyla...
Gene silencing has evolved in a broad range of organisms probably as defense mechanisms against inva...
g.oxfordjournals.org/ D ow nloaded from 2 Despite the fact that 45 % of all human gene promoters do ...
International audienceDouble-stranded RNAs (dsRNAs) and their 'diced' small RNA products can guide k...
RNA polymerase III (Pol III) as well as Pol II (35S) promoters are able to drive hairpin RNA (hpRNA)...
AbstractMethylation of cytosine in the DNA inhibits the transcription by RNA polymerase II in higher...
AbstractMethylation of cytosine-residues in the sequence CpG affects the expression of many genes an...
The use of nucleases to probe the higher-order organisation of genes and the development of cell-fre...
International audienceMethylation has often been correlated with transcriptional inhibition of genes...
Mammalian genomes contain huge numbers of short interspersed elements (SINEs). An extreme case is p...
The methylation sensitive restriction enzymes Hha I and Hpa II were used to analyse the methylation ...
[[abstract]]In this communication we identify and initially characterize two antagonistic activities...
Summary A hallmark of vertebrate genes is that actively transcribed genes are hypomethylated in crit...
Epigenetic gene silencing results from the inhibition of transcription or from posttranscriptional R...
Eukaryotic genomes contain 5-methylcytosine (5mC) as a rare base. 5mC arises by postsynthetic modifi...
AbstractMany RNA polymerase II- or III-transcribed genes are inactive when their promoter is methyla...
Gene silencing has evolved in a broad range of organisms probably as defense mechanisms against inva...
g.oxfordjournals.org/ D ow nloaded from 2 Despite the fact that 45 % of all human gene promoters do ...
International audienceDouble-stranded RNAs (dsRNAs) and their 'diced' small RNA products can guide k...
RNA polymerase III (Pol III) as well as Pol II (35S) promoters are able to drive hairpin RNA (hpRNA)...