Investigating the Susceptibility and Death Kinetic of Pseudomonas Aeruginosa Bacterium Standard and Clinical Strains to the Copper Oxide Nanoparticle

Microbial resistance is considered as one of the challenges in coping with infectious diseases. Thus, to find or synthesize new antimicrobial substances is very important. Copper oxide (CuO) has drawn the attention of the researchers due to its antibacterial effect in coping with microbial resistance. In this research, the antibacterial effects of copper oxide nanoparticles on the standard and clinical strains of Pseudomonas aeruginosa, which its new strains have been associated with microbial resistance, were examined and compared. In this experimental-interventional research, a copper oxide nanoparticle was synthesized at a size of 33 nm using copper sulfate chemical reduction method. Then, the antibacterial effects of copper oxide nanoparticle on standard strain (ATCC 27853) and clinical strain of Pseudomonas aeruginosa were examined using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and bacteria death kinetics. Data derived from investigating the bacteria death kinetics were analyzed using statistical tests at a significant level of p value (