Effect of blood purification on serum miR-126 and VEGF levels in the process of atherosclerosis in uremic patients under maintenance hemodialysis

This study aims to study the influence of different blood purification approaches on the expression of miR-126 and VEGF serum levels in the development of atherosclerosis in uremic patients under maintenance hemodialysis (MHD). A total of 207 MHD patients with uremia were divided into HD (hemodialysis, n = 60), HDF (hemodiafiltration, n = 72), and HD + HP (hemodialysis + hemoperfusion, n = 75) groups based on different purification approaches. Eighty individuals who underwent physical examinations during the same period constituted the healthy controls. The serum levels of miR-126 and VEGF were examined by qRT-PCR and ELISA both before and after treatment, and the intima media thickness (IMT) value and plaque area were evaluated by color Doppler ultrasound. The serum miR-126 level was down-regulated in MHD patients compared with healthy controls, and this was negatively linked to VEGF. The post-treatment expression level of serum miR-126 in the HDF and HD + HP groups was remarkably increased, but VEGF was decreased in MHD patients, and especially significantly in the HDF group. In addition, IMT and plaque area were obviously improved in the HD group after treatment. Pearson correlation analysis showed a negative correlation of miR-126 with IMT and plaque area, but a positive association between VEGF and IMT and plaque area. miR-126 and VEGF are expected to become a valuable biomarker for monitoring the progression of atherosclerosis in uremic patients undergoing MHD. Keywords: Blood purification, Maintenance hemodialysis, Uremia, miR-126, VEG