Inhibition of dihydropyridine-sensitive calcium channels by the plant alkaloid ryanodine

AbstractAt micromolar concentrations, ryanodine interacts with the dihydropyridine receptor of rabbit skeletal muscle transverse tubules. Ryanodine displaces specifically bound [3H]PN200-110 with an apparent inhibition constant of approx. 95 μM and inhibits dihydropyridine-sensitive calcium channels in the same preparation with an IC50 of approx. 45 μM. These concentrations of ryanodine are approximately three orders of magnitude higher than those required to saturate binding of the alkaloid to the ryanodine receptor of sarcoplasmic reticulum and to open the calcium release channel of sarcoplasmic reticulum (i.e. 20 nM (1988) J. Gen. Physiol. 92, 1–26). Thus at sufficiently high dose, ryanodine may affect SR as well as plasma membrane Ca permeabilities