Effects of Phoenix dactylifera pollen grains extract supplementation on post-thaw quality of Arabian stallion semen

This study explored the effect of extender supplementation with different concentrations of date palm pollen grain (DPPG) on post-thawed sperm motility, viability index, membrane and acrosome integri-ties in Arabian stallions. Five ejaculates from each of four Arabian stallions were subjected to cryo-preservation with a modified INRA-82, without any supplementation (control) or supplemented with 50, 100, 150, 200 and 250 mg DPPG. After thawing, all samples were maintained at 37 oC, while analyses were performed at 0, 1, and 2 and 3 hours. Sperm motility percentage, viability index, mem-brane integrity percentage and acrosome integrity of each sample were determined by conventional laboratory methods. The addition of 100 mg DPPG resulted in improved maintenance of sperm moti¬lity after 0 and 60 min post-thawing, as compared to the control and other treatment groups. Non-significant effects on viability index were observed after enrichment of extender with 100 and 150 mg DPPG. The addition of 100 and 150 mg DPPG resulted in significant (P