Apoptosis gene profiling reveals spatio-temporal regulated expression of the p53/Mdm2 pathway during lens development
- Geatrell, Jenny C.
- Mui (Iryn) Gan, Peng
- Mansergh, Fiona C.
- Kisiswa, Lilian
- Jarrin, Miguel
- Williams, Llinos A.
- Evans, Martin J.
- Boulton, Mike E.
- Wride, Michael A.
DOIAbstract
AbstractEvidence is emerging for apoptosis gene expression in the lens during development. Therefore, here we used a filter array to assess expression of 243 apoptosis-related genes in the developing postnatal mouse lens using 33P labelled cDNA synthesized from p7 and p14 mouse lenses. We demonstrated that 161 apoptosis-related genes were expressed at levels significantly above background and 20 genes were potentially significantly differentially expressed (P<0.05) by at least 2-fold between p7 and p14. We used RT-PCR to confirm expression of these genes in newborn, p7, p14 and 4 wk mouse lens cDNA samples. Expression of 19/20 of the genes examined was confirmed, while 5 genes (Huntingtin, Mdm2, Dffa, galectin-3 and Mcl-1) were confirmed as...
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