Effect of Yizhitongxuan decoction on learning and memory ability, Gαq/11 expression and Na+−K+-ATP enzyme activity in rat models of Alzheimer's Disease

AbstractObjectiveTo study the effects of Yizhitongxuan decoction on learning and memory abilities, Gαq/11 expression and Na+−K+−ATP enzyme activity in rat models of Alzheimer's disease (AD) caused by injecting Aβ25-35 into the hippocampus.MethodsNinety male Wistar rats (age ≤10 months) were selected and injected with Aβ25-35 into their hippocampi to establish model animals, which were randomly divided into six groups including a sham-operated group (blank group), a model group, a donepezil HCL group (Western Medicine group), and a high/general/dilute concentrations of Yizhitongxuan decoction groups (TCM I II III group). The Morris water maze was used to examine the learning and memory abilities of rats in each group by place navigation and spatial probe tests. Then, the rats were sacrificed to collect the hippocampi for biochemical tests, using western blotting to detect the expression of Gαq/11 and an ultramicro Na +−K+−ATP enzyme kit to measure Na+−K+−ATP enzyme activity.ResultsYizhitongxuan decoction improved model rats' learning and memory abilities, and increased the expression of Gαq/11 in the hippocampus and the level of Na+−K+−ATP enzyme activity in brain tissue.ConclusionsYizhitongxuan decoction could improve model rats' learning and memory abilities, and had a regulating effect on the expression of Gαq/11 and Na+−K+−ATP enzyme activity