Heterogeneity in an isolated membrane protein Has the ‘authentic cytochrome oxidase’ been identified?

AbstractThe criteria of homogeneity or native state of a protein are prone to become ambiguous when applied to membrane proteins, such as cytochrome-c oxidase, which are purified by extraction with detergents. Properties of the purified material depend on the detergent used and on details of the purification protocol followed with any single batch of a preparation. We present arguments to show that the evidence presently available in published form does not justify the designation [(1987) J. Biol. Chem. 262 3160–3164] of one type of preparation as being closer to the native state than others