The stability of quantitative blood count parameters using the ADVIA 2120i hematology analyzer

AbstractObjectivesTo evaluate the stability of complete blood count (CBC) parameters by measuring at multiple time points up to 72h after venepuncture.Methods36 samples submitted for routine haematology were measured at 0, 8, 24, 48, 72h. 18 samples were kept at room temperature (23–25°C) and the other 18 at 4°C. The stability of the CBC parameters was determined by comparing the results to the 0h sample. White Blood Cell Count (WBC), Hemoglobin (Hb) concentration, Red Blood Cell (RBC) count, Hematocrit (Hct), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Cellular Hemoglobin Concentration Mean (CHCM), Mean Platelet Volume (MPV), RBC distribution width (RDW), Platelet (PLT) and Reticulocyte counts were studied.ResultsMost parameters were stable for 24h at 4°C, except for MCV, MCHC, CHCM and MPV. MCV and MPV increased after 8h (p<0.0001), whereas MCHC and CHCM decreased significantly after 8h. Significant changes were found for MCHC in samples kept at 4°C for 48h (p=0.002), and for CHCM kept for 72h (p