Presence of B cells within aortic valves in patients with aortic stenosis: Relation to severity of the disease

AbstractBackgroundAortic stenosis (AS) shares several similarities with atherosclerosis. Recent reports showed that B cells are implicated in atherosclerosis progression through macrophage–B cells bidirectional interaction.We aimed to study the in loco presence of B cells within aortic valves and to determine its modulators.MethodsThirty-seven patients with severe AS were studied. Immunohistochemistry was performed on valve leaflets using antibodies against CD20, B cell-activating factor of the tumor necrosis factor family receptor (BAFF-R) and CD68. Plasma inflammatory markers were also determined.ResultsThe B cells were detected within aortic leaflets from 5 to 31/mm2 (17.9±11.6/mm2). Double-staining showed that 27±13.5% of B cells express BAFF-R. There were positive correlations between the number of B cells and macrophages (r=0.45, p=0.018), and between macrophages and B cell-associated BAFF-R expression (r=0.66, p=0.002). The number of B cells was associated with the valve calcification (r=0.41, p=0.039), and with the maximum transvalvular gradient (r=0.63, p=0.02). The BAFF-R expression was positively correlated with maximum transvalvular gradient (r=0.39, p=0.031) and negatively with aortic valve area (r=−0.41, p=0.048). There were no correlations between the number of B cells and plasma markers.ConclusionsIt might be hypothesized that, like in atherosclerosis, increasing number of B cells within aortic valves may accelerate inflammation and thus potentiate the progression of AS