Increased renal calcium and magnesium transporter abundance in streptozotocin-induced diabetes mellitus
- Lee, C-T.
- Lien, Y-H.H.
- Lai, L-W.
- Chen, J-B.
- Lin, C-R.
- Chen, H-C.
DOIAbstract
Diabetes is associated with renal calcium and magnesium wasting, but the molecular mechanisms of these defects are unknown. We measured renal calcium and magnesium handling and investigated the effects of diabetes on calcium and magnesium transporters in the thick ascending limb and distal convoluted tubule in streptozotocin (STZ)-induced diabetic rats. Rats were killed 2 weeks after inducing diabetes, gene expression of calcium and magnesium transporters in the kidney was determined by real-time polymerase chain reaction, and the abundance of protein was assessed by immunoblotting. Our results showed that diabetic rats had significant increase in the fractional excretion for calcium and magnesium (both P<0.01), but not for sodium. Reverse ...
Add to ORKG