Increase in CO3H— influx and cellular pH in glucose-stimulated pancreatic islets

AbstractWhen rat islets are preincubated with fluorescein diacetate and examined in a spectrofluorometer, the intracellular pH rapidly increases by 0.21 pH units in response to a rise from 1.7 – 16.8 mM glucose. This coincides with a marked increase in 14Co3H— net uptake by the islets, suggesting that the glucose-induced increase in H+ generation rate is compensated for by stimulation of CO3 H—/Cl− exchange