New fluorescence of nonenzymatically glucosylated human serum albumin

AbstractGlucosylated human serum albumin (G-HSA) obtained under incubation with glucose at 37° C for 8 days showed a new fluorescence with a maximum at 430 nm, resulting in quenching of the fluorescence of only one tryptophan residue on HSA. The quantum yield of new fluorescence is 0.024 at 25° C. The analysis of the excitation spectra allowed us to conclude the absence of energy transfer. In G-HSA, non-disulfide cross-linking hexamer was confirmed by SDS-PAGE