Nucleotide sequence of a reiterated rat DNA fragment

AbstractA reiterated component of rat DNA was isolated by restriction with HindIII endonuclease and polyacrylamide gel electrophoresis. Sequence analysis revealed that the fragment was 179 nucleotides long. Unlike the known 370N reiterated rat DNA fragment which contained a high m5C content (2.7 mole%), this repetitive element contained a rather low m5C content (0.5 mole%). The present rat repetitive sequence appeared to be of α-type as shown by its significant homologies with α DNA sequenes of African green monkey and human. The possibility of sequence heterogeneity is discussed