Cytosolic free Ca2+ in isolated rat hepatocytes as measured by quin2 Effects of noradrenaline and vasopressin

AbstractCytosolic [Ca2+] has been measured by using the Ca2+-sensitive indicator quin2 in rat liver cells. Optimal loading and hydrolysis have been obtained by equilibrating the cells with 50 μM quin2 acetoxymethyl ester for 150 s. The increase in [Ca2+]i initiated by noradrenaline and vasopressin was reduced but not abolished by removing external Ca2+