Study of cell kinetics by computer-analyzed flow cytometric histograms

AbstractA mathematical procedure for analyzing the cell proliferation kinetics from DNA content histograms, measured by flow cytometry, is presented. This procedure is based on a cell cycle model which uses a continuity equation for the S-phase transit. Four tumor celllines in culture were studied. A human melanoma line (JR01) was examined in exponential growth. For another human melanoma (M14) and a human astrocytoma (DF) lines, a sequence of flow cytometric histgrams with the corresponding growth curve was processed, determining the rate of DNA synthesis and the S-phase cell influx. The same kinetic parameters were evaluated also in a murine tumor line (3LL C108) treated with an antineoplastic drug (ICRF 159)