Bioethanol Production from Oil Palm Frond by Simultaneous Saccharification and Fermentation

AbstractEthanol production from oil palm frond (OPF) by simultaneous saccharification and Saccharomyces cerevisiae TISTR5048 fermentation was investigated. Solid fraction of OPF (20% TS) was pretreated by 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking at room temperature for 24hours. Pretreated OPF by presoaking in 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 contained 37%, 42% and 49% of cellulose, respectively. Pretreated OPF was simultaneous saccharification by cellulase enzyme (Cellic CTec2, Novozymes) and sequentially fermentation. Sugar concentration in OPF cellulose hydrolysis of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 45.72, 55.73 and 56.94g/l, respectively. Ethanol concentration of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 14.5, 15.0 and 17.2g/L, respectively. 2% NaOH in H2O2 presoaking was the best pretreatment with 82.11% of total solids recovery and containing 49.9% of cellulose with enzyme digestion ability of 37.6%