P48 Integrated analysis of genomic and transcriptomic data in clear-cell renal cell carcinoma

Renal cell carcinoma (RCC) is the most widespread kidney tumor, which originates mostly from distal kidney tubules. RCC is the major mortality cause in excretory system cancer in adults, and constitutes about 80% of various kidney cancers. The 5-year survival rate is 60–70%, but lowers significantly in case of metastasis formation. The tumor is relatively resistant to chemotherapy and radiation therapy, but responds to immunotherapy. Target drugs (e.g. sunitinib, bevacizumab, α-interferon, sorafenib) are more preferable in RCC therapy. In most cases RCC is caused by obesity, smoking, arterial hypertension and hereditary factors.4 patients of Voronezh Regional Clinical Oncology Center, aged from 50 to 75years old were enrolled in this study. All were diagnosed with renal cell carcinoma confirmed by immunohistochemical analysis.Gene expression profiling was performed using Affymetrix GeneAtlas system with Affymetrix Hunam Gene 1.1 ST DNA- microarrays. Data moralization, statistical analysis and differentially expressed genes (DEG) list creation were performed using Partek Genomics Suite v. 6.6. DNA samples were sequenced on Ion Proton sequencer with Comprehensive Cancer Panel primer pool. Subsequent pathway analysis and biological interpretation were conducted using Ingenuity Pathway Analysis system and Ion Reporter Suite.3528 genes were differentially expressed with expression change more than 2 times, and 351 genes changed their expression more than 5 times (p-value