Background/purposePrimary tooth endodontic filling materials should be bio-compatible with periodontal tissue. The purpose of this study was to analyze the biologic effects of different endodontic filling materials for primary teeth on a human osteosarcoma cell line (U2OS).Materials and methodsExperimental groups comprised different mixes of endodontic filling materials: zinc oxide-eugenol (ZnOE) + formocresol (FC); calcium hydroxide [Ca(OH)2] + FC; Ca(OH)2 + iodoform + deionized water; Ca(OH)2 + iodoform +camphorated parachlorophenol (CPC); Ca(OH)2 + CPC; and Vitapex. These were prepared and used to fill special glass rings, which were subsequently eluted in 10 mL of cell culture medium at 37ºC in a 5% carbon dioxide-in-air atmosphere for ...