The effect and action mechanism of resveratrol on the vascular endothelial cell by high glucose treatment

AbstractTo investigate the effect and action mechanism of resveratrol on the vascular endothelial cell by high glucose treatment. Primarily cultured human umbilical vein endothelial cells (HUVECs) were pretreated by resveratrol (0.2μmol/L) and holding for 6h, and then cultured in Dulbecco Modified Eagle Medium (DMEM) within 0.45mmol/L of palmimte acid and 32.8mmol/L of glucose, which is holding for 12h. The cells were collected to analyze the expression of E-selected element. Supernatant of cultured cells, induced by 100nmol/L insulin for 30min, was used to analyze the nitric oxide content. Compared with normal control cells, the secretion of nitric oxide is stimulated by insulin decrease, however, the expression of E-selected element increased in HUVEC. Resveratrol treatment increased the secretion of nitric oxide stimulated by insulin and decreased the expression of E-selected element and partly counteracts the impairment of high glucose and palmitate acid on the function of endothelial cells. Resveratrol can improve and protect the function of high glucose and fatty acid cultured endothelial cell, and therefore may be a promising medicine in the prevention or therapy of diabetic macrovascular diseases