The East Asian summer monsoon (EASM) variability on orbital timescale has been extensively investigated in Quaternary loess and speleothems. However, EASM variability during pre-Quaternary time remains poorly understood. Here, we report a continuous upper Miocene cyclostratigraphic record from lake deposits of the Tianshui Basin, Northeast Tibet, to reconstruct past variations of the regional hydrological cycle. Our results, combined with previously published cyclostratigraphic records from Northeast Tibet, show that regional lake expansion cycles have been consistently dominated by ~100 kyr eccentricity forcing over most of the middle to late Miocene. These ~100 kyr cycles corroborate a significant forcing of the East Asian hydrological cy...