CORRECTIVE EFFECT OF THIOTRIAZOLINE ON TOXICITY AND CONDITION OF PRO- AND ANTIOXIDANT HOMEOSTASIS IN RATS AGAINST TOXIC ACTION OF NANOCHROMIUM CITRATE

The aim of the study. Researching a corrective effect of thiotriazoline on general toxicity and parameters of biochemical detoxication under conditions of action of nanochromium citrate toxic doses. Materials and Methods. The effect of thiotriazoline (100 mg/kg) on nanochromium citrate (NCC) toxicity and condition of pro- and antioxidant homeostasis against a single injection of sublethal (DL0) and lethal (DL50, DL100) doses of the compound (3 mg/kg, 4,5 mg/kg, 5 mg/kg) is examined in an acute experiment on laboratory rats. The content of Malone aldehyde (MA), reduced glutathione (Г-SH) and catalase activity (CA) was detected in the erythrocytes. General antioxidant activity (GAA), the content of oxidizing-modied proteins (OMP) and free SH-groups were detected in the blood plasma. The content of MA, Г-SH, OMP and CA was studied in the post-nuclear supernatants of the liver homogenates. Results and Discussion. Administration of thiotriazoline with therapeutic purpose against sublethal and lethal doses of NCC is found to decrease considerably the signs of intoxication and increase survival of animals. Since untreated animals died on the 2-3 days, under thiotriazoline action it occurred on the 6-8 days. Intoxication of animals with high doses of NCC is accompanied by intensication of the processes of free radical lipid oxidation and protein oxidation modication in the body, reduced activity of catalase, general antioxidant activity of the blood plasma, content of free SH-groups and reduced glutathione. The action of thiotriazoline against minimally toxic dose of NCC (3 mg/kg) appeared to be more effective as compared to untreated animals antioxidant activity in the blood plasma increased reliably without noticeable changes of SH-groups content. Conclusions. A prolonged administration of thiotriazoline decreased the signs of intoxication and lethal outcome of animals against toxic doses of nanochromium citrate in an acute experiment. Thiotriazoline prevents the development of disorders of oxidizing-antioxidizing homeostasis in the body of intoxicated animals, although, a single injection of thiotriazoline against lethal doses of nanochromium citrate is less effective