CHARACTER OF THE STRUCTURAL RESPONSE OF LONG BONES OF OUT-OF-BREED LABORATORY FEMALE RATS TO THE EFFECT OF EXPERIMENTAL HYPERGLYCEMIA

Summary. Research of the influence of structural reaction of tissues and organs on disorders of homeostasis and development of steady functional malformation of system character remains an important direction in modern medicine. The aim of the work – to investigate the structural features of the long bones of female rats in the modeling of streptozotocin inducing diabetes mellitus. Material and Methods. The experiment was carried out on 14 white out-of-breed female rats aged 2.4–2.6 months and 180–200 g of weight. All animals were divided into control and experimental animals. The control animals were in typical vivarium conditions. Streptozotocin inducing diabetes mellitus was modeled according to Kikhtyak-Skripnik method. The duration of the experiment was 30 days. Results and Discussion. A high level of glucose in the blood provokes the development of a wide range of pathological changes. The conducted studies of the spongy substance and proximal epiphyseal cartilage of femurs fix the lag of the osteometric parameters of the experimental animals. The length of bones is less than control by 0.06 %, the deficit of the width of the proximal epiphysis reaches 0.05%. The decrease in osteoblast productivity and metabolic processes in bone tissue is manifested by a decrease in the amount of primary and total spongiosis. Conclusions. Experimental streptozotocin-induced diabetes mellitus is the cause of development of dystrophic-atrophic changes and slowing of growth processes in the femurs of experimental white out-of-breed laboratory female rats. There is a decrease in the osteometric parameters of the femurs, the width of the proximal epiphyseal cartilage and its constituent components. Histologically, the detected dystrophic changes in the zone of indifferent cartilaginous cells, in the zones of proliferation and destruction, are combined with an increasing of interstitial substance in all areas. In the spongy substance, we detected a decrease in the number of osteoblasts, the volume of primary and general spongiosis substance